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Christmas tissue culture rota

Monday 19th DecKelly6E,F
Wednesday 21st DecCristina6E,F
Friday 23rd DecCristina6E,F
Monday 26th DecCecile6E,F,insect
Wednesday 28th DecAdam6E, F, Insect
Friday 30th Dec



Monday 2nd JanCecile6E,F,insect
Wednesday 4th JanCristina6E,F


For fire or first aid dial "3333"

Security: dial "4444"


Lab Databases



Cell Bank:

HO numbers:

Lab plasmids:

Reagent database:

BSF Wright Lab folder:

Freezer contents

-80 Freezer:

-20 Freezers and Fridges:

Emergency freezers in L/M1 link corridor (also E021, F019). Emergency fridge in F019.

Freezer alarm contact list and alarm reprorgamming: Steve Howarth

What to do if you are ill and can't come to work:

Booking annual leave (Flexileave): 

Lone worker policy:

Parcels awaiting collection

Stores order form

Stores catalogue

TC Hood Booking

TC Hood Booking B/L/146A (insect cell culture room). You will need to email Simon Grist to get added to this booking system:

Travelling - fill out travel log for insurance:

Useful email addresses

If you would like to send out an email request to the biology department for reagents, plasmids etc, you can use the following email groups:

Lab responsibilities

Last updated: 17/09/2021

Adam Enzymes (PCR, RE), pH meter, molecular biology, gel tanks, CL3 protocols and handbook regular revision reminders
CecileElectronic and physical repository (databases, plasmids), tissue culture room fridge
EnricaHome Office regulated procedures database, microscopes
DelphineFreezers, Lab meeting rota
JoePlate reader, ensure tissue culture incubator water reservoirs full
CristinaOrdering, cleaning and jobs rota, cell lines, thawing fresh 293 cells, underbench -80 freezer, AKTA Pures


Protein press

Cleaning rota for L1 communal lab areas

Last updated:  



for 2022

Chemical and

Sonicator room

Cold room

30 degree 


37 degree


Gel doc and

centrifuge area

28th January

Team 30
25th February

Team 30
25th MarchTeam 30

29th April
Team 30

27th May

Team 30

24th June

Team 30
29th July

Team 30
26th AugustTeam 30

30th September
Team 30

28th October

Team 30

25th November

Team 30
16th December

Team 30

Cleaning of these areas will normally be come between 14:00 and 16:00 to avoid disrupting work in these areas.

Lab meeting rota

Last updated: 17/09/2021

16.09.2211:00JarrodB/K/157ALab meeting
23.09.2211:00CecileWilliamson RoomsJournal Club
30.09.2211:00EnricaWilliamson RoomsJournal Club


Williamson RoomsJournal Club
14.10.2211:00VincentWilliamson RoomsLab meeting
21.10.2211:00DelphineWilliamson RoomsLab meeting
28.10.2211:00AdamWilliamson RoomsJournal Club
No lab meeting - Parasite@York meeting



Williamson RoomsLab meeting
18.11.2211:00EnricaWilliamson RoomsLab meeting
25.11.2211:00No lab meeting - Parasite@York meeting

02.12.2211:00CristinaWilliamson RoomsLab meeting
09.12.2211:00VincentWilliamson RoomsJournal Club
No lab meeting - Parasite@York meeting

No lab meeting - Parasite@York meeting

13.01.2311:00KellyWilliamson RoomsLab meeting
20.01.2311:00JoWilliamson RoomsLab meeting
No lab meeting - Parasite@York meeting

Health and Safety

Last updated: 18/10/2021

York H&S web site:

Safety monitor for L1 is Christoph Baumann

L1 Induction and Rules

Risk assesments and SOP's and general safety

Hard copies of Risk Assessments and training records are kept in the lab and on this wiki

Generic department risk assessment for working with chemicals:

Overarching "good lab practice" document at York:

L1 and BSF labs GM and biological safety RA for working with CL2 kinetoplastids:

L1 labs Code of Practice working with trypanosomes (SAPO):

CL1 GM work (cloning and protein expression in HEK cells):

CL3 work in H block (Code of practice, risk assessment, SOPs):

SAPO licence:


Lab coat and gloves should be worn when undertaking any procedure in the lab.

Safety glasses can be found in the last bay in the top drawer under the plate shaker and should be worn when handling strong acids and bases, fuming and corrosive substances.

A full face shield must be used when handling liquid nitrogen and when taking out heated liquid from the microwave oven. The fume hood is located in the chemical room.

Waste routes

Waste disposal:


First aid

First aid room is in biology atrium near mail room.


Fire sweeper for L1: 

Spills, accidents, incidents and near misses 

Chemical spills:

The chemical spill kit and mercury spill kit are located under the main lab sink.

There is a further chemical spill kit and safety signs located near the stores window. Before clearing hazardous spills, check the MSDS for any special measures.

In the event of a mercury spill, evacuate the area for 30 minutes, don protective clothing and respiratory mask before clearing spill.

The mop and bucket are located in the cell culture room.

First aid kits are located in both the office and lab corridors of L1 in the middle of the building. 

Accidents/incidents and near misses can be reported 

Accident/Incident and near miss reporting: "It is important that the Campus Health and Safety Service are made aware of any incident on the Campus that has lead to someone being injured or made ill, or to property or equipment being damaged. We also need to know about those incidents that could have led to someone being injured or made ill, or to property or equipment being damaged - a 'near miss'.

Competency tables

Last updated: 01/10/2022

Training records can be found from historical electronic copies of this page

TechniquePerson responsibleProtocolRisk assesmentPerformTrain others
AKTA Pure handling (HisTrap purification, column cleaning/regeneration)Cristina

AKTA pure HisTrap purification_clean and recharge


Cecile, Delphine, Cristina, AdamCecile, Delphine, Cristina, Adam
Avexis screenEnricaAVEXIS  AVEXIS RACecile, Enrica, Cristina, AdamCecile, Enrica
Cecile, AdamCecile
Batch his-tag purificationDelphineBatch his-tag purification
Cecile, Delphine, Cristina, AdamCecile, Delphine, Cristina, Adam
BIAcore handling

Cecile, Enrica, Cristina, AdamCecile
Bioluminescence assay (plate reader)Delphine

Blood smearsDelphine

Chemical deglycosylation of proteins

DNA gel electrophoresis (agarose/EtBr/SybrSafe)JoeDNA ElectrophoresisDNA Electrophoresis RAAdam, Cecile, Delphine, Enrica, Cristina, VincentAdam, Cecile, Delphine, Enrica, Cristina, Vincent
DNA/plasmid prepJoePlasmid PreparationPlasmid Preparation RAAdam, Cecile, Delphine, Enrica, Cristina, VincentAdam, Cecile, Delphine, Enrica, Cristina, Vincent
ELISA (BIO-proteins)Cecile
Adam, Cecile, Delphine, Enrica, CristinaCecile, Enrica, Delphine
EZ-linked biotinylationCecileEZ-link biotinylation protocol.docx

Adam, CecileCecile
Flow cytometry

Adam, Enrica, VincentTraining provided by TF staff
Freezing/thawing cellsEnricaProtocol
Adam, Cecile, Delphine, EnricaCecile, Enrica, Adam
Fumigating hoodsCristinaProtocol

HEK293 (E, F, 6E) cell culture (incl. transfection)Enrica
Adam, Cecile, Delphine, Enrica, Cristina, VincentCecile, Enrica
Hybridoma generation (fusion, culture, screen)Cecile

I.P. injection mouseDelphineI.P injection
Cecile, Delphine, Enrica-
Delphine, Enrica, AdamEnrica
Incubator cleaningCristinaProtocol

In vitro fertilisation (mouse)Enrica
Insect cell culture and transfectionVincent

Cristina, VincentCristina, Vincent
IVISDelphineIVIS imaging
Leishmania cultureAdamLeishmania RAAdamAdam
Lentivirus production/ transductionAdam



Lentivirus RAAdamAdam
Magnetic beads purificationAdam
Adam, DelphineAdam
Microbiology hood cleaning

Mouse oocyte harvestingEnricaMouse oocyte harvesting
Myeloma/hybridoma cell cultureCecile

OX68 expression and purificationGavinProtocol

Parasite isolation from bloodDelphine

Collecting blood using cardiac puncture

Parasite isolation from blood

PCR/DNA digest/ligationVincent

Adam, Cecile, Enrica, Cristina, VincentAdam, Cecile, Enrica, Cristina, Vincent
Percutaneous infection (mouse)Cecile
Plasmid data upload to lab databaseEnrica

N/AEnrica, Adam, CecileEnrica, Adam, Cecile
Plasmid transformation of competent E. coliKellyAdam, Cecile, Delphine, Enrica, Cristina, VincentAdam, Cecile, Delphine, Enrica, Cristina, Vincent
Prey normalisation (beta lactamase)EnricaPrey normalisation
Cecile, Enrica, CristinaCecile, Enrica
Preparing chemically competent bacteriaCristina

Protein concentration quantificationCristina

Cecile, Delphine, Enrica, Cristina, AdamCecile, Enrica
Protein dialysis/concentrationCecile

Cecile, Delphine, Enrica, AdamCecile, Enrica, Adam
Protein pressCecile
Cecile, CristinaCecile

Adam, Cecile, Delphine, Enrica, VincentAdam, Cecile, Delphine, Enrica, Vincent

Adam, Cecile, Delphine, Enrica, VincentCecile, Enrica, Adam
Subcutaneous injection mouseDelphine
Size exclusion chromatographyCristinaSuperdex™ 200 Increase 10/300 chromatographySize exclusion and AKTA Pure RA

Cecile, Cristina, AdamCecile, Cristina, Adam
Tissue cultureCristinaProtocol
Adam, Cecile, Delphine, Enrica, Vincent, JoeAdam, Cecile, Delphine, Enrica, Vincent
Western blotVincent

Adam, Cecile, Delphine, VincentCecile, Enrica, Vincent, Adam
I.V injectionDelphine

Vectors at Geneart and Twist

Last updated: 16/09/2015

Twist ref:Geneart ref:Our ref:Database ref:
N/Avector1_C819_Mero-bio (no insert)V
N/AC820type II signalpeptide-bio-linker-Cd4V
v1_SP-HLBio-Cd4p1943_E668type II signalpeptide-his-linker-bio-Cd4V75
v2_Cd4-BioLHp0821_D725rat CD200 - Cd4- bio-linker-his


v4_mero-Cd4-COMPBLFHV087_MTRAP_M770Mero (MTRAP) - Cd4-COMP-beta-lac-3xFLAG-His*V87
v3_mero-Cd4-BioLHI078Mero (RH1) Cd4-bio-Linker-his*V74
v6_mero-BioLHP3054_Q942Mero (RH5-N-term)-bio-Linker-his* (no Cd4)V
v5_Cd4-COMPBLFHN/A- Cd4-COMP-beta-lac-3xFLAG-His*V


R-drive connection

Network path for Team 30 drive is R:\rsrch\gjw515\lab\T30_Sanger

To map the R drive to a Sanger issued laptop. Sign into EduRoam with your York username and password. Open filed explorer and click map network drive and map the following path.  Check the box regarding using an alterate credentials. 


Check the box regarding using an alternate credentials. 

Log in using "ITSYORK\(Your user name)

Plasmid database templates

New plasmid upload spreadsheet:

Example template for plasmids P6360 to P6368:

Feature annotatoin spreadsheet:

Social media

The Wright Lab Facebook group can be found on the following link - please sign up!

Equipment info

Galaxy R incubator, Wolf labs

Current access code for this incubator is 0000.

In case access code is misplaced, to change the access code on this inbubator, follow instructions below:

  1. Press DIAG then ENG
  2. Key in 1973 press ENTER
  3. Tab down to
  5. Then press RESET
  6. Press EXIT several times to return back to main screen

That will now have removed the pin code from the incubator.

To connect to your TECAN folder:

Important! You will first need to install IT Services' VPN connector called Pulse Secure.  Instructions on how to do this are at

  • Windows 10
    • Open File Explorer (press the "Windows" key and E, or type File Explorer in the search box)
    • Click "This PC"
    • Click "Computer" tab at top and then "Map network drive"
    • Select drive letter R
    • In folder box enter:  \\\username
    • Do NOT tick the 'Reconnect at Login' box, but DO tick the 'Connect using different credentials' box and then click Finish
    • In the "Enter Network Credentials" box, click "More choices", then "Use a different account"
    • Enter user name in the form ITSYORK\usernameand then your Uni password.  If you are using your own login account on your own PC or laptop, then tick the "Remember my credentials" box.  Do NOT tick this box if it is possible for someone else to login to your account on your PC or laptop.
    • Click OK.

Technology Facility

To register:

1. Go to this link and log-in with your standard university account

2. Press the button to "register" and then follow the prompts for registering. Note that when answering the prompt of what PI/lab you are under, you should search "gjw515" as Gavin's name may not appear.

3. Once you're done registering, use the same link to make bookings for equipment throughout the Technology Facility ( Under the "Instruments" tab you can tick the boxes next to pieces of equipment you want to view/book, then on the calendar click and drag to reserve a time on that piece of equipment. To finalize your booking you'll need to select a Work Order out of the drop-down list we set up of the lab's standard grants.

4. It's recommended that for your first booking you should email the facility head (for example, Peter O'Toole for cytometry) to schedule a training session. These are mostly for your own benefit, as unlike Sanger there's no further approval forms you need to do post-training. 


Security01904323333 or 3333 from campus phone
Dr Christoph Baumann

L1 Safety

Dr James
Dr Marie-Christine Labarthe-Last CL2/CL3 lab manager

Lucy Hudson


David 8524
University H&S services
Ext. 2020
Ext. 3333

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