YP3 poses three high-level research questions (RQs) in pyrenoid biology and self-assembly physics:
RQ1. What is the physics of equilibrium and active self-assembly recruited in photosynthetic pyrenoids that drives the formation and dissolution of their complex substructures?
RQ2. Does pyrenoid self-assembly in different organisms recruit the same class of biophysical phenomena, and are components interchangeable?
RQ3. What is the minimal number of components required to assemble a synthetic functional pyrenoid, and can it be realised as an artificial biotechnology?